Last data update: May 20, 2024. (Total: 46824 publications since 2009)
Records 1-4 (of 4 Records) |
Query Trace: Moser BD[original query] |
---|
Pattern of antimicrobial susceptibility obtained from blood isolates of a rare but emerging human pathogen, Gordonia polyisoprenivorans
Moser BD , Pellegrini GJ , Lasker BA , Brown JM . Antimicrob Agents Chemother 2012 56 (9) 4991-3 The genus Gordonia, originally described in 1971 by Tsukamura, consisted of both clinical and environmental isolates (9).... |
Nocardia amikacinitolerans sp. nov., an amikacin-resistant human pathogen.
Ezeoke I , Klenk HP , Potter G , Schumann P , Moser BD , Lasker BA , Nicholson A , Brown JM . Int J Syst Evol Microbiol 2012 63 1056-1061 Five isolates from clinical human sources were evaluated. Analysis of the near full length 16S rRNA gene showed 99.9-100 % similarity among the strains. The results of a comparative phylogenetic analysis of the 16S rRNA gene sequences indicated that the isolates belonged to the genus Nocardia. Phenotypic and molecular analyses were performed on the clinical isolates. Traditional phenotypic analyses included morphologic, biochemical/physiological, chemotaxonomic and antimicrobial susceptibility profiling. Molecular studies included 1441-bp 16S rRNA and 1246-bp gyrB gene sequence analyses, as well as DNA-DNA hybridizations. Biochemical analysis failed to differentiate the putative novel species from its phylogenetic neighbors; however, molecular studies were able to distinguish the patient strains and confirm them as a single species. Based on 16S rRNA gene sequence analysis, similarity between the isolates and their closest relatives (Nocardia araoensis, Nocardia arthritidis, Nocardia beijingensis and Nocardia niwae) were less than or equal to 99.3 %. Partial gyrB gene sequence analysis showed 98-99.7 % relatedness among the isolates. Nocardia lijiangensis and Nocardia xishanensis were the isolates' closest related species based on gyrB gene sequence analysis and showed 95.7 and 95.3 % similarity, respectively. Resistance to amikacin and molecular analyses, including DNA-DNA hybridization, distinguished the five patient strains from their phylogenetic neighbors, and the results of this polyphasic study indicated a novel species of Nocardia for which we propose the name Nocardia amikacinitolerans sp. nov., with strain W9988T (=DSM 45539 T = CCUG 59655T) as the type strain. |
Mycobacterium chelonae-abscessus complex associated with sinopulmonary disease, northeastern USA
Simmon KE , Brown-Elliott BA , Ridge PG , Durtschi JD , Mann LB , Slechta ES , Steigerwalt AG , Moser BD , Whitney AM , Brown JM , Voelkerding KV , McGowan KL , Reilly AF , Kirn TJ , Butler WR , Edelstein PH , Wallace RJ Jr , Petti CA . Emerg Infect Dis 2011 17 (9) 1692-700 Members of the Mycobacterium chelonae-abscessus complex represent Mycobacterium species that cause invasive infections in immunocompetent and immunocompromised hosts. We report the detection of a new pathogen that had been misidentified as M. chelonae with an atypical antimicrobial drug susceptibility profile. The discovery prompted a multicenter investigation of 26 patients. Almost all patients were from the northeastern United States, and most had underlying sinus or pulmonary disease. Infected patients had clinical features similar to those with M. abscessus infections. Taxonomically, the new pathogen shared molecular identity with members of the M. chelonae-abscessus complex. Multilocus DNA target sequencing, DNA-DNA hybridization, and deep multilocus sequencing (43 full-length genes) support a new taxon for these microorganisms. Because most isolates originated in Pennsylvania, we propose the name M. franklinii sp. nov. This investigation underscores the need for accurate identification of Mycobacterium spp. to detect new pathogens implicated in human disease. |
Nocardia niwae sp. nov., isolated from human pulmonary sources.
Moser BD , Klenk HP , Schumann P , Potter G , Lasker BA , Steigerwalt AG , Hinrikson HP , Brown JM . Int J Syst Evol Microbiol 2010 61 438-442 Members of the genus Nocardia are responsible for cutaneous, pulmonary and disseminated human infections. From 2003 to 2008, four nocardioform strains (W8027, W8681, W9071, W9241T) were isolated from persons in the state of Florida, USA. Ribosomal gene sequencing analysis suggested that a novel Nocardia species had been isolated. These strains underwent polyphasic taxonomic analysis. Phenotypic analyses included morphologic examination, biochemical profiling and antimicrobial susceptibility testing. Molecular studies included 16S rRNA and DNA gyrase B subunit (gyrB) gene sequence analyses and DNA-DNA hybridization. Phylogenetic neighbours were determined through 16S rRNA and gyrB gene sequence analyses. Differential phenotypic characteristics of the novel Nocardia species compared to phylogenetically related species were growth at 45C and 3 out of 4 novel strains utilized L-rhamnose. The antimicrobial profiles could not reliably distinguish the novel species from related nocardiae. Analysis showed that the 16S rRNA gene sequences of the four novel isolates were identical. The BLAST analysis of the near full length 16S rRNA gene showed 99.2 % sequence similarity to N. araoensis DSM 44729T, N. arthritidis DSM 44731T and N. beijingensis JCM 10666 T, 98.7 % to N. amamiensis DSM 45066T, 98.2 % to N. pneumoniae JCM 12119T and 97.8 % to N. takedensis JCM 13313T; the analysis of partial gyrB gene sequences showed 95.4 % similarity to N. arthritidis DSM 44731T, 95.3 % to N. gamkensis DSM 44956T, 94.4 % to N. pneumoniae JCM 12119T, 93.8 % to asiatica DSM44668T, 93.5 % to N. amamiensis DSM 45066T, 93.4 % to N. beijingensis JCM 10666 T and 93.2 % to N. araoensis DSM 44729T. The DNA-DNA hybridization percentages among the four novel strains were 86-89 %; the hybridization percentages of W9241T compared to N. beijingensis JCM 10666T was 47 %, to N. araoensis DSM 44729T was 46 %, to N. arthritidis DSM 44731T was 44 %, to N. amamiensis DSM 45066T was 32 % and to N. asiatica DSM 44668T was 20 %. The results of our polyphasic taxonomic analysis suggested that a novel species of Nocardia was identified for which we propose the name Nocardia niwae sp. nov. The type strain is W9241T ( = DSM 45340T = CCUG 57756T). |
- Page last reviewed:Feb 1, 2024
- Page last updated:May 20, 2024
- Content source:
- Powered by CDC PHGKB Infrastructure